Separation of 3,4-Benzpyrene Metabolites by Paper Partition Chromatography

The quantitative separation of estrogens by paper partition chromatography.

The varying success of known chromat’ographic procedures for the separation and identification of microquantities of estrogens (1, 2) has led to the development of paper partit!ion chromatographic techniques which are reported here. These t’echniques permit rapid separation, identification, and quantitative estimation of free est.rogens in biological materials. The solvent systems of o-dichloro...

Separation of Plant Pigments by Paper Chromatography

Anyone who has ever played on grass or had an unplanned encounter with a bush knows that plants contain pigments which adhere to varying degrees to fabric (and skin!). The fact that a small industry has risen up in aid of attempts to remove stains like these and many others shows that not all of the molecules can be treated in the same way if their removal is desired. Part of the reason behind ...

Separation of Amino Acids by Paper Chromatography

Chromatography is a common technique for separating chemical substances. The prefix “chroma,” which suggests “color,” comes from the fact that some of the earliest applications of chromatography were to separate components of the green pigment, chlorophyll. You may have already used this method to separate the colored components in ink. In this experiment you will use chromatography to separate...

Paper Chromatography: Separation and

The example of column chromatography (Figure 1) demonstrates the typical features found in this analytical technique. The diagram shows an experiment where a two-component mixture is subjected to column chromatography. The column is packed with a solid material called the stationary phase. A liquid solvent or eluting solution is poured into the column and completely wets the solid packing mater...

Study of phenylketonuria by paper chromatography